Part:BBa_K258101:Design

PrtE domain of ABC transporter of Erwinia chrysanthemi

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1136
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1136
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1136
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1136
  Illegal AgeI site found at 1270
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The gene actually will be fully function with other two genes which are PrtD and PrtF.

Source

E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 50 kd protein to the prtD gene product (protein PrtD).

References

[1]Protease secretion by Erwinia chrysanthemi: the specific secretion functions are analogous to those of Escherichia coli c!-haemolysin Sylvie Letoffe, Philippe Delepelaire and Cecile Wandersman Unite de Gdnetique Moleculaire (URA CNRS 1149), Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France The EMBO Journal vol.9 no.5 pp. 1 375 - 1382, 1990
[2]http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=D19F08CB014&log%24=protalign&blast_rank=1&list_uids=41549